Can more than one person still login to the same account using Two-Factor authentication

Yes, you can.

When at the Two-Factor authentication setup page you would need all people that require access to this account to scan the QR code or add the secret key to their authenticator app.

Setup details can be found here: Logging in for the first time with Two-Factor Authentication (2FA)

If you have already setup your 2FA for just 1 person to access, you must reset the 2FA for that account to start the process again so all people can scan the QR code or add the secret key to their authenticator app. See: Resetting Two-Factor Authentication (2FA) for an admin account